› 3gtd

X-ray diffraction
2.4Å resolution

2.4 Angstrom crystal structure of fumarate hydratase from Rickettsia prowazekii

Released:
DOI: 10.2210/pdb3gtd/pdb
PDB entry 3gtd coloured by chain, front view.
PDB entry 3gtd coloured by chain, side view.
PDB entry 3gtd coloured by chain, top view.
Source organism: Rickettsia prowazekii str. Madrid E
Primary publication:
Structure of fumarate hydratase from Rickettsia prowazekii, the agent of typhus and suspected relative of the mitochondria.
Phan I, Subramanian S, Olsen C, Edwards TE, Guo W, Zhang Y, Van Voorhis WC, Stewart LJ, Myler PJ
Acta Crystallogr Sect F Struct Biol Cryst Commun 67 1123-8 (2011)
PMID: 21904061

Function and Biology Details

Reaction catalysed: 
(S)-malate = fumarate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195621 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fumarate hydratase class II Chains: A, B
Molecule details ›
Chains: A, B
Length: 482 amino acids
Theoretical weight: 53.36 KDa
Source organism: Rickettsia prowazekii str. Madrid E
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9ZCQ4 (Residues: 1-461; Coverage: 100%)
Gene names: RP665, fumC
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MLI 2 x MLI
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P3121
Unit cell:
a: 144.9Å b: 144.9Å c: 106.21Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.194 0.193 0.226
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Structural, biochemical and biophysical characterization of recombinant human fumarate hydratase.
Ajalla Aleixo et al. (2019)
1 more

2 mentions without citation

Rapid catalytic template searching as an enzyme function prediction procedure.
Nilmeier et al. (2013)
1 more