› 3guc

X-ray diffraction
2.25Å resolution

Human Ubiquitin-activating Enzyme 5 in Complex with AMPPNP

Released:
DOI: 10.2210/pdb3guc/pdb
PDB entry 3guc coloured by chain, front view.
PDB entry 3guc coloured by chain, side view.
PDB entry 3guc coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Walker JR, Bacik JP, Li Y, Weigelt J, Bountra C, Arrowsmith CH, Edwards AM, Bochkarev A, Dhe-Paganon S, Structural Genomics Consortium (SGC)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190465 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin-like modifier-activating enzyme 5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 292 amino acids
Theoretical weight: 32.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9GZZ9 (Residues: 57-329; Coverage: 68%)
Gene names: UBA5, UBE1DC1
Sequence domains: ThiF family
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand ANP 1 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P3221
Unit cell:
a: 77.394Å b: 77.394Å c: 205.892Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.203 0.255
Expression system: Escherichia coli BL21(DE3)

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