› 3gv7

X-ray diffraction
2.2Å resolution

Human DNA polymerase iota in complex with T template DNA and incoming dTTP

Released:
DOI: 10.2210/pdb3gv7/pdb
PDB entry 3gv7 coloured by chain, front view.
PDB entry 3gv7 coloured by chain, side view.
PDB entry 3gv7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of error-prone replication and stalling at a thymine base by human DNA polymerase iota.
Kirouac KN, Ling H
EMBO J 28 1644-54 (2009)
PMID: 19440206

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116342 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase iota Chain: B
Molecule details ›
Chain: B
Length: 420 amino acids
Theoretical weight: 47.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UNA4 (Residues: 26-445; Coverage: 57%)
Gene names: POLI, RAD30B
Sequence domains:
Structure domains:
5'-D(P*AP*TP*GP*GP*GP*TP*CP*CP*T)-3' Chain: T
Molecule details ›
Chain: T
Length: 9 nucleotides
Theoretical weight: 2.75 KDa
Source organism: Homo sapiens
Expression system: Not provided
5'-D(*AP*GP*GP*AP*CP*CP*C)-3' Chain: P
Molecule details ›
Chain: P
Length: 7 nucleotides
Theoretical weight: 2.11 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand TTP 1 x TTP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P6522
Unit cell:
a: 97.19Å b: 97.19Å c: 201.94Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 0.209 0.258
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

12 review citations

Y-family DNA polymerases and their role in tolerance of cellular DNA damage.
Sale et al. (2012)
11 more

3 mentions without citation

Structural basis of error-prone replication and stalling at a thymine base by human DNA polymerase iota.
Kirouac et al. (2009)
2 more