› 3gvj

X-ray diffraction
1.48Å resolution

Crystal structure of an endo-neuraminidaseNF mutant

Released:
DOI: 10.2210/pdb3gvj/pdb
PDB entry 3gvj coloured by chain, front view.
PDB entry 3gvj coloured by chain, side view.
PDB entry 3gvj coloured by chain, top view.
Source organism: Escherichia phage K1F
Primary publication:
Structural basis for the recognition and cleavage of polysialic acid by the bacteriophage K1F tailspike protein EndoNF.
Schulz EC, Schwarzer D, Frank M, Stummeyer K, Mühlenhoff M, Dickmanns A, Gerardy-Schahn R, Ficner R
J Mol Biol 397 341-51 (2010)
PMID: 20096705

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (2->8)-alpha-sialosyl linkages in oligo- or poly(sialic) acids
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170081 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Mature tail spike protein Chain: A
Molecule details ›
Chain: A
Length: 670 amino acids
Theoretical weight: 74.46 KDa
Source organism: Escherichia phage K1F
Expression system: Escherichia coli
UniProt:
  • Canonical: Q04830 (Residues: 246-910; Coverage: 63%)
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: SLB, SIA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: SLB, SIA
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: R3
Unit cell:
a: 119.09Å b: 119.09Å c: 175.98Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.194 0.216
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Sialic acids in the brain: gangliosides and polysialic acid in nervous system development, stability, disease, and regeneration.
Schnaar et al. (2014)
7 more

2 mentions without citation

The potential antiviral effect of major royal jelly protein2 and its isoform X1 against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2): Insight on their sialidase activity and molecular docking.
Habashy et al. (2020)
1 more