› 3gwl

X-ray diffraction
2.1Å resolution

Crystal structure of ASFV pB119L, a viral sulfhydryl oxidase

Released:
DOI: 10.2210/pdb3gwl/pdb
PDB entry 3gwl coloured by chain, front view.
PDB entry 3gwl coloured by chain, side view.
PDB entry 3gwl coloured by chain, top view.
Source organism: African swine fever virus BA71V
Primary publication:
Dimer interface migration in a viral sulfhydryl oxidase.
Hakim M, Fass D
J Mol Biol 391 758-68 (2009)
PMID: 19576902

Function and Biology Details

Reaction catalysed: 
2 R'C(R)SH + O(2) = R'C(R)S-S(R)CR' + H(2)O(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179180 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FAD-linked sulfhydryl oxidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 106 amino acids
Theoretical weight: 12.91 KDa
Source organism: African swine fever virus BA71V
Expression system: Escherichia coli
UniProt:
  • Canonical: Q65163 (Residues: 1-103; Coverage: 87%)
Gene names: 9GL, B119L, Ba71V-073
Sequence domains: Erv1 / Alr family
Structure domains: ERV/ALR sulfhydryl oxidase domain

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P212121
Unit cell:
a: 46.45Å b: 69Å c: 83.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.218 0.261
Expression system: Escherichia coli

Quick links

Citations

8 review citations

The rapidly expanding universe of giant viruses: Mimivirus, Pandoravirus, Pithovirus and Mollivirus.
Abergel et al. (2015)
7 more

6 mentions without citation

Chemistry and Enzymology of Disulfide Cross-Linking in Proteins.
Fass et al. (2018)
5 more