› 3gx7

X-ray diffraction
2.95Å resolution

Crystal structure of the T. tengcongensis SAM-I riboswitch variant U34C/A94G mutant A6C/U7G/A87C/U88G bound with SAM

Released:
DOI: 10.2210/pdb3gx7/pdb
Model geometry
Fit model/data
PDB entry 3gx7 coloured by chain, front view.
PDB entry 3gx7 coloured by chain, side view.
PDB entry 3gx7 coloured by chain, top view.
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis
Primary publication:
Discrimination between closely related cellular metabolites by the SAM-I riboswitch.
Montange RK, Mondragón E, van Tyne D, Garst AD, Ceres P, Batey RT
J Mol Biol 396 761-72 (2010)
PMID: 20006621

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-303236 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
RNA (94-MER) Chain: A
Molecule details ›
Chain: A
Length: 94 nucleotides
Theoretical weight: 30.6 KDa
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis
Expression system: Not provided
Sequence domains: SAM riboswitch aptamer (S box leader)

Ligands and Environments

2 bound ligands:
Ligand SAM 1 x SAM
Ligand MG 6 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: RIGAKU RU200
Spacegroup: P43212
Unit cell:
a: 62.63Å b: 62.63Å c: 159.615Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.214 0.271
Expression system: Not provided

Quick links

Citations

8 review citations

RNA Structural Dynamics As Captured by Molecular Simulations: A Comprehensive Overview.
Šponer et al. (2018)
7 more

4 mentions without citation

Using RNA Sequence and Structure for the Prediction of Riboswitch Aptamer: A Comprehensive Review of Available Software and Tools.
Antunes et al. (2017)
3 more