› 3gxy

X-ray diffraction
2.4Å resolution

Crystal structure of cyanovirin-n complexed to a synthetic hexamannoside

Released:
DOI: 10.2210/pdb3gxy/pdb
PDB entry 3gxy coloured by chain, front view.
PDB entry 3gxy coloured by chain, side view.
PDB entry 3gxy coloured by chain, top view.
Source organism: Nostoc ellipsosporum
Primary publication:
Structures of the complexes of a potent anti-HIV protein cyanovirin-N and high mannose oligosaccharides.
Botos I, O'Keefe BR, Shenoy SR, Cartner LK, Ratner DM, Seeberger PH, Boyd MR, Wlodawer A
J Biol Chem 277 34336-42 (2002)
PMID: 12110688

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160490 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Cyanovirin-N Chains: A, B
Molecule details ›
Chains: A, B
Length: 101 amino acids
Theoretical weight: 11.02 KDa
Source organism: Nostoc ellipsosporum
Expression system: Escherichia coli
UniProt:
  • Canonical: P81180 (Residues: 1-101; Coverage: 100%)
Sequence domains: CVNH domain
Structure domains: Cyanovirin-N

Ligands and Environments

Carbohydrate polymer : NEW Components: OPM, MAN
Ligand OPM 2 x OPM
Ligand MAN 4 x MAN
2 bound ligands:
Ligand NHE 1 x NHE
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: P41212
Unit cell:
a: 61.352Å b: 61.352Å c: 147.568Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.208 0.271
Expression system: Escherichia coli

Quick links

Citations

20 review citations

Targeting the glycans of glycoproteins: a novel paradigm for antiviral therapy.
Balzarini J. (2007)
19 more

3 mentions without citation

Mannose-Specific Lectins from Marine Algae: Diverse Structural Scaffolds Associated to Common Virucidal and Anti-Cancer Properties.
Barre et al. (2019)
2 more