› 3gyb

X-ray diffraction
1.6Å resolution

Crystal structure of a LacI-family transcriptional regulatory protein from Corynebacterium glutamicum

Released:
DOI: 10.2210/pdb3gyb/pdb
PDB entry 3gyb coloured by chain, front view.
PDB entry 3gyb coloured by chain, side view.
PDB entry 3gyb coloured by chain, top view.
Source organism: Corynebacterium glutamicum
Entry authors: Palani K, Burley SK, Swaminathan S, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185674 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH lacI-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 280 amino acids
Theoretical weight: 30.6 KDa
Source organism: Corynebacterium glutamicum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NTY1 (Residues: 62-330; Coverage: 81%)
Gene name: Cgl0170
Sequence domains: Periplasmic binding protein-like domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P1
Unit cell:
a: 38.073Å b: 61.882Å c: 65.604Å
α: 115.83° β: 106.37° γ: 90.5°
R-values:
R R work R free
0.219 0.215 0.231
Expression system: Escherichia coli

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