› 3h05

X-ray diffraction
1.65Å resolution

The Crystal Structure of a Putative Nicotinate-nucleotide Adenylyltransferase from Vibrio parahaemolyticus

Released:
DOI: 10.2210/pdb3h05/pdb
PDB entry 3h05 coloured by chain, front view.
PDB entry 3h05 coloured by chain, side view.
PDB entry 3h05 coloured by chain, top view.
Source organism: Vibrio parahaemolyticus
Entry authors: Stein AJ, Cuff ME, Sather A, Shackelford G, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
ATP + beta-nicotinate-D-ribonucleotide = diphosphate + deamido-NAD(+)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183327 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
nicotinate-nucleotide adenylyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 177 amino acids
Theoretical weight: 20.01 KDa
Source organism: Vibrio parahaemolyticus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q87J40 (Residues: 1-177; Coverage: 100%)
Gene name: VPA0413
Sequence domains: Cytidylyltransferase-like
Structure domains: HUPs

Ligands and Environments

1 bound ligand:
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 41.206Å b: 70.722Å c: 130.495Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.194 0.214
Expression system: Escherichia coli

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