› 3h08

X-ray diffraction
1.6Å resolution

Crystal structure of the Ribonuclease H1 from Chlorobium tepidum

Released:
DOI: 10.2210/pdb3h08/pdb
PDB entry 3h08 coloured by chain, front view.
PDB entry 3h08 coloured by chain, side view.
PDB entry 3h08 coloured by chain, top view.
Source organism: Chlorobaculum tepidum
Primary publication:
Structure, stability, and folding of ribonuclease H1 from the moderately thermophilic Chlorobium tepidum: comparison with thermophilic and mesophilic homologues.
Ratcliff K, Corn J, Marqusee S
Biochemistry 48 5890-8 (2009)
PMID: 19408959

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188128 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease H Chains: A, B
Molecule details ›
Chains: A, B
Length: 146 amino acids
Theoretical weight: 16.33 KDa
Source organism: Chlorobaculum tepidum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q93SU7 (Residues: 1-146; Coverage: 100%)
Gene names: rnh, rnhA
Sequence domains: RNase H
Structure domains: Ribonuclease H-like superfamily/Ribonuclease H

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1, ALS BEAMLINE 8.3.1
Spacegroup: P1
Unit cell:
a: 33.457Å b: 33.388Å c: 66.072Å
α: 100.38° β: 99.43° γ: 90.82°
R-values:
R R work R free
0.161 0.16 0.194
Expression system: Escherichia coli

Quick links

Citations

1 review citation

HIV-1 Reverse Transcriptase/Integrase Dual Inhibitors: A Review of Recent Advances and Structure-activity Relationship Studies.
Mahboubi-Rabbani et al. (2021)
14 other articles

8 mentions without citation

What lessons can be learned from studying the folding of homologous proteins?
Nickson et al. (2010)
7 more