3h0g

X-ray diffraction
3.65Å resolution

RNA Polymerase II from Schizosaccharomyces pombe

Released:
Primary publication:
Schizosacharomyces pombe RNA polymerase II at 3.6-A resolution.
Proc Natl Acad Sci U S A 106 9185-90 (2009)
PMID: 19458260

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-126928 (preferred)
Entry contents:
12 distinct polypeptide molecules
Macromolecules (12 distinct):
DNA-directed RNA polymerase II subunit RPB3 Chains: C, O
Molecule details ›
Chains: C, O
Length: 297 amino acids
Theoretical weight: 33.75 KDa
Source organism: Schizosaccharomyces pombe 972h-
UniProt:
  • Canonical: P37382 (Residues: 1-297; Coverage: 100%)
Gene names: SPCC1442.10c, rpb3
Sequence domains:
Structure domains:
DNA-directed RNA polymerase II subunit rpb4 Chains: D, P
Molecule details ›
Chains: D, P
Length: 135 amino acids
Theoretical weight: 15.38 KDa
Source organism: Schizosaccharomyces pombe 972h-
UniProt:
  • Canonical: O74825 (Residues: 1-135; Coverage: 100%)
Gene names: SPBC337.14, rpb4
Sequence domains: RNA polymerase Rpb4
Structure domains: Growth Hormone; Chain: A;
DNA-directed RNA polymerases I, II, and III subunit RPABC1 Chains: E, Q
Molecule details ›
Chains: E, Q
Length: 210 amino acids
Theoretical weight: 23.95 KDa
Source organism: Schizosaccharomyces pombe 972h-
UniProt:
  • Canonical: Q09191 (Residues: 1-210; Coverage: 100%)
Gene names: SPAC23C4.15, rpb5
Sequence domains:
Structure domains:
DNA-directed RNA polymerases I, II, and III subunit RPABC2 Chains: F, R
Molecule details ›
Chains: F, R
Length: 142 amino acids
Theoretical weight: 15.74 KDa
Source organism: Schizosaccharomyces pombe 972h-
UniProt:
  • Canonical: P36595 (Residues: 1-142; Coverage: 100%)
Gene names: SPCC1020.04c, rpb6, rpo15
Sequence domains: RNA polymerase Rpb6
Structure domains: Eukaryotic RPB6 RNA polymerase subunit
DNA-directed RNA polymerase II subunit rpb7 Chains: G, S
Molecule details ›
Chains: G, S
Length: 172 amino acids
Theoretical weight: 19.12 KDa
Source organism: Schizosaccharomyces pombe 972h-
UniProt:
  • Canonical: O14459 (Residues: 1-172; Coverage: 100%)
Gene names: SPACUNK4.06c, SPAPYUK71.02, rpb7
Sequence domains:
Structure domains:
DNA-directed RNA polymerases I, II, and III subunit RPABC3 Chains: H, T
Molecule details ›
Chains: H, T
Length: 125 amino acids
Theoretical weight: 14.32 KDa
Source organism: Schizosaccharomyces pombe 972h-
UniProt:
  • Canonical: Q92399 (Residues: 1-125; Coverage: 100%)
Gene names: SPBC14C8.12, rpb8
Sequence domains: RNA polymerase Rpb8
Structure domains: Nucleic acid-binding proteins
DNA-directed RNA polymerase II subunit RPB9 Chains: I, U
Molecule details ›
Chains: I, U
Length: 113 amino acids
Theoretical weight: 13.19 KDa
Source organism: Schizosaccharomyces pombe 972h-
UniProt:
  • Canonical: O74635 (Residues: 1-113; Coverage: 100%)
Gene names: SPAPYUG7.04c, rpb9
Sequence domains:
Structure domains: N-terminal domain of TfIIb
DNA-directed RNA polymerases I, II, and III subunit RPABC5 Chains: J, V
Molecule details ›
Chains: J, V
Length: 71 amino acids
Theoretical weight: 8.29 KDa
Source organism: Schizosaccharomyces pombe 972h-
UniProt:
  • Canonical: O13877 (Residues: 1-71; Coverage: 100%)
Gene names: SPAC1B3.12c, rpb10
Sequence domains: RNA polymerases N / 8 kDa subunit
Structure domains: Homeodomain-like
DNA-directed RNA polymerase II subunit RPB11 Chains: K, W
Molecule details ›
Chains: K, W
Length: 123 amino acids
Theoretical weight: 14.14 KDa
Source organism: Schizosaccharomyces pombe 972h-
UniProt:
  • Canonical: P87123 (Residues: 1-123; Coverage: 100%)
Gene names: SPAC3A12.07, rpb11
Sequence domains:
Structure domains: RNA polymerase, RBP11-like subunit
DNA-directed RNA polymerases I, II, and III subunit RPABC4 Chains: L, X
Molecule details ›
Chains: L, X
Length: 63 amino acids
Theoretical weight: 7.22 KDa
Source organism: Schizosaccharomyces pombe 972h-
UniProt:
  • Canonical: P48011 (Residues: 1-63; Coverage: 100%)
Gene names: SPBC19C2.03, rpb12, rpc10
Sequence domains: DNA directed RNA polymerase, 7 kDa subunit
Structure domains: RNA polymerase ii, chain L

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 163.034Å b: 202.684Å c: 391.258Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.297 0.297 0.321