› 3h0g

X-ray diffraction
3.65Å resolution

RNA Polymerase II from Schizosaccharomyces pombe

Released:
DOI: 10.2210/pdb3h0g/pdb
Model geometry
Fit model/data
PDB entry 3h0g coloured by chain, front view.
PDB entry 3h0g coloured by chain, side view.
PDB entry 3h0g coloured by chain, top view.
Source organism: Schizosaccharomyces pombe 972h-
Primary publication:
Schizosacharomyces pombe RNA polymerase II at 3.6-A resolution.
Spåhr H, Calero G, Bushnell DA, Kornberg RD
Proc Natl Acad Sci U S A 106 9185-90 (2009)
PMID: 19458260

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126928 (preferred)
Entry contents:
12 distinct polypeptide molecules
Macromolecules (12 distinct):
DNA-directed RNA polymerase II subunit rpb1 Chains: A, M
Molecule details ›
Chains: A, M
Length: 1752 amino acids
Theoretical weight: 194.37 KDa
Source organism: Schizosaccharomyces pombe 972h-
UniProt:
  • Canonical: P36594 (Residues: 1-1752; Coverage: 100%)
Gene names: SPBC28F2.12, rpb1
Sequence domains:
Structure domains:
DNA-directed RNA polymerase II subunit RPB2 Chains: B, N
Molecule details ›
Chains: B, N
Length: 1210 amino acids
Theoretical weight: 138.03 KDa
Source organism: Schizosaccharomyces pombe 972h-
UniProt:
  • Canonical: Q02061 (Residues: 1-1210; Coverage: 100%)
Gene names: SPAC23G3.01, SPAC521.06, rpb2
Sequence domains:
Structure domains:
DNA-directed RNA polymerase II subunit RPB3 Chains: C, O
DNA-directed RNA polymerase II subunit rpb4 Chains: D, P
DNA-directed RNA polymerases I, II, and III subunit RPABC1 Chains: E, Q
DNA-directed RNA polymerases I, II, and III subunit RPABC2 Chains: F, R
DNA-directed RNA polymerase II subunit rpb7 Chains: G, S
DNA-directed RNA polymerases I, II, and III subunit RPABC3 Chains: H, T
DNA-directed RNA polymerase II subunit RPB9 Chains: I, U
DNA-directed RNA polymerases I, II, and III subunit RPABC5 Chains: J, V
DNA-directed RNA polymerase II subunit RPB11 Chains: K, W
DNA-directed RNA polymerases I, II, and III subunit RPABC4 Chains: L, X

Ligands and Environments

2 bound ligands:
Ligand ZN 16 x ZN
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 163.034Å b: 202.684Å c: 391.258Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.297 0.297 0.321

Quick links

Citations

6 review citations

Distinct Mechanisms of Transcription Initiation by RNA Polymerases I and II.
Engel et al. (2018)
5 more

14 mentions without citation

Basic mechanisms of RNA polymerase II activity and alteration of gene expression in Saccharomyces cerevisiae.
Kaplan CD. (2013)
13 more