› 3h0u

X-ray diffraction
1.5Å resolution

Crystal structure of a putative enoyl-CoA hydratase from Streptomyces avermitilis

Released:
DOI: 10.2210/pdb3h0u/pdb
PDB entry 3h0u coloured by chain, front view.
PDB entry 3h0u coloured by chain, side view.
PDB entry 3h0u coloured by chain, top view.
Source organism: Streptomyces avermitilis
Entry authors: Bonanno JB, Freeman J, Bain KT, Miller S, Romero R, Wasserman S, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo trimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-182679 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enoyl-CoA hydratase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 289 amino acids
Theoretical weight: 30.87 KDa
Source organism: Streptomyces avermitilis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q82Q85 (Residues: 8-285; Coverage: 98%)
Gene names: SAVERM_635, echA2
Sequence domains: Enoyl-CoA hydratase/isomerase
Structure domains: 2-enoyl-CoA Hydratase; Chain A, domain 1

Ligands and Environments

2 bound ligands:
Ligand DMS 7 x DMS
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: C2
Unit cell:
a: 134.896Å b: 102.442Å c: 96.173Å
α: 90° β: 132.11° γ: 90°
R-values:
R R work R free
0.16 0.159 0.176
Expression system: Escherichia coli

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