› 3h20

X-ray diffraction
1.99Å resolution

Crystal structure of primase RepB'

Released:
DOI: 10.2210/pdb3h20/pdb
PDB entry 3h20 coloured by chain, front view.
PDB entry 3h20 coloured by chain, side view.
PDB entry 3h20 coloured by chain, top view.
Source organism: Plasmid RSF1010
Primary publication:
Structure and function of primase RepB' encoded by broad-host-range plasmid RSF1010 that replicates exclusively in leading-strand mode.
Geibel S, Banchenko S, Engel M, Lanka E, Saenger W
Proc Natl Acad Sci U S A 106 7810-5 (2009)
PMID: 19416864

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-176002 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RepB-like DNA primase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 323 amino acids
Theoretical weight: 35.96 KDa
Source organism: Plasmid RSF1010
Expression system: Escherichia coli
UniProt:
  • Canonical: Q52349 (Residues: 1-323; Coverage: 100%)
Gene name: repB
Sequence domains: RepB DNA-primase from phage plasmid
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand DPO 1 x DPO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P43212
Unit cell:
a: 91.74Å b: 91.74Å c: 83.48Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.195 0.232
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Breaking and joining single-stranded DNA: the HUH endonuclease superfamily.
Chandler et al. (2013)
4 more

4 mentions without citation

Primase-polymerases are a functionally diverse superfamily of replication and repair enzymes.
Guilliam et al. (2015)
3 more