› 3h2b

X-ray diffraction
2Å resolution

Crystal structure of the SAM-dependent methyltransferase cg3271 from Corynebacterium glutamicum in complex with S-adenosyl-L-homocysteine and pyrophosphate. Northeast Structural Genomics Consortium Target CgR113A

Released:
DOI: 10.2210/pdb3h2b/pdb
PDB entry 3h2b coloured by chain, front view.
PDB entry 3h2b coloured by chain, side view.
PDB entry 3h2b coloured by chain, top view.
Source organism: Corynebacterium glutamicum ATCC 13032
Entry authors: Forouhar F, Lew S, Seetharaman J, Sahdev S, Xiao R, Foote EL, Ciccosanti C, Wang D, Everett JK, Nair R, Acton TB, Rost B, Montelione GT, Hunt JF, Tong L, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163600 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SAM-dependent methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 203 amino acids
Theoretical weight: 22.25 KDa
Source organism: Corynebacterium glutamicum ATCC 13032
Expression system: Escherichia coli
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

2 bound ligands:
Ligand SAH 2 x SAH
Ligand PPV 1 x PPV
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P212121
Unit cell:
a: 70.827Å b: 72.424Å c: 74.826Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.173 0.226
Expression system: Escherichia coli

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