› 3h36

X-ray diffraction
1.8Å resolution

Structure of an uncharacterized domain in polyribonucleotide nucleotidyltransferase from Streptococcus mutans UA159

Released:
DOI: 10.2210/pdb3h36/pdb
PDB entry 3h36 coloured by chain, front view.
PDB entry 3h36 coloured by chain, side view.
PDB entry 3h36 coloured by chain, top view.
Source organism: Streptococcus mutans
Entry authors: Cuff ME, Hatzos C, Jedrzejczak R, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184255 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyribonucleotide nucleotidyltransferase Chain: A
Molecule details ›
Chain: A
Length: 93 amino acids
Theoretical weight: 10.87 KDa
Source organism: Streptococcus mutans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8DWB2 (Residues: 231-320; Coverage: 12%)
Gene names: SMU_155, pnp
Sequence domains: Polyribonucleotide nucleotidyltransferase, RNA binding domain
Structure domains: Polyribonucleotide nucleotidyltransferase, RNA-binding domain

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand EDO 1 x EDO
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P6122
Unit cell:
a: 59.312Å b: 59.312Å c: 93.09Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.201 0.199 0.236
Expression system: Escherichia coli

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