› 3h3n

X-ray diffraction
1.73Å resolution

Glycerol Kinase H232R with Glycerol

Released:
DOI: 10.2210/pdb3h3n/pdb
PDB entry 3h3n coloured by chain, front view.
PDB entry 3h3n coloured by chain, side view.
PDB entry 3h3n coloured by chain, top view.
Source organism: Enterococcus casseliflavus
Primary publication:
Structural characterizations of glycerol kinase: unraveling phosphorylation-induced long-range activation.
Yeh JI, Kettering R, Saxl R, Bourand A, Darbon E, Joly N, Briozzo P, Deutscher J
Biochemistry 48 346-56 (2009)
PMID: 19102629

Function and Biology Details

Reaction catalysed: 
ATP + glycerol = ADP + sn-glycerol 3-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-128519 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycerol kinase Chains: O, X
Molecule details ›
Chains: O, X
Length: 506 amino acids
Theoretical weight: 55.83 KDa
Source organism: Enterococcus casseliflavus
Expression system: Escherichia coli
UniProt:
  • Canonical: O34153 (Residues: 1-506; Coverage: 100%)
Gene name: glpK
Sequence domains:
Structure domains: Nucleotidyltransferase; domain 5

Ligands and Environments

3 bound ligands:
Ligand GOL 2 x GOL
Ligand EDO 6 x EDO
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21212
Unit cell:
a: 96.812Å b: 200.464Å c: 56.505Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.207 0.216
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The bacterial phosphoenolpyruvate:carbohydrate phosphotransferase system: regulation by protein phosphorylation and phosphorylation-dependent protein-protein interactions.
Deutscher et al. (2014)
1 more

3 mentions without citation

The FGGY carbohydrate kinase family: insights into the evolution of functional specificities.
Zhang et al. (2011)
2 more