› 3h41

X-ray diffraction
1.79Å resolution

Function and Biology Details

Reaction catalysed: 
The enzyme releases L-Ala-gamma-D-Glu dipeptides from cell wall peptides via cleavage of an L-Ala-gamma-D-Glu-|-L-Lys bond.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180939 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gamma-D-glutamyl-L-lysine dipeptidyl-peptidase Chain: A
Molecule details ›
Chain: A
Length: 311 amino acids
Theoretical weight: 35.17 KDa
Source organism: Bacillus cereus ATCC 10987
Expression system: Escherichia coli
UniProt:
  • Canonical: Q736M3 (Residues: 24-333; Coverage: 100%)
Gene names: BCE_2878, ykfC
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ALA 1 x ALA
Ligand DGL 1 x DGL
Ligand PO4 1 x PO4
Ligand PG4 6 x PG4
2 modified residues:
Ligand MSE 5 x MSE
Ligand OCS 1 x OCS

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: C2
Unit cell:
a: 95.205Å b: 59.373Å c: 61.333Å
α: 90° β: 103.27° γ: 90°
R-values:
R R work R free
0.165 0.163 0.197
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Peptidoglycan NlpC/P60 peptidases in bacterial physiology and host interactions.
Griffin et al. (2023)
23 other articles

20 mentions without citation

Cell Wall Hydrolases in Bacteria: Insight on the Diversity of Cell Wall Amidases, Glycosidases and Peptidases Toward Peptidoglycan.
Vermassen et al. (2019)
19 more