› 3h4l

X-ray diffraction
2.5Å resolution

Crystal Structure of N terminal domain of a DNA repair protein

Released:
DOI: 10.2210/pdb3h4l/pdb
PDB entry 3h4l coloured by chain, front view.
PDB entry 3h4l coloured by chain, side view.
PDB entry 3h4l coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Functional residues on the surface of the N-terminal domain of yeast Pms1.
Arana ME, Holmes SF, Fortune JM, Moon AF, Pedersen LC, Kunkel TA
DNA Repair (Amst) 9 448-57 (2010)
PMID: 20138591

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146887 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA mismatch repair protein PMS1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 367 amino acids
Theoretical weight: 41.44 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P14242 (Residues: 1-365; Coverage: 42%)
Gene names: N2317, PMS1, YNL082W
Sequence domains: DNA mismatch repair protein, C-terminal domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ANP 2 x ANP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P212121
Unit cell:
a: 75.592Å b: 103.288Å c: 117.167Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.235 0.286
Expression system: Escherichia coli

Quick links

Citations

4 review citations

DNA repair mechanisms and the bypass of DNA damage in Saccharomyces cerevisiae.
Boiteux et al. (2013)
3 more

6 mentions without citation

New insights into the mechanism of DNA mismatch repair.
Reyes et al. (2015)
5 more