› 3h4w

X-ray diffraction
1.5Å resolution

Structure of a Ca+2 dependent Phosphatidylinositol-specific phospholipase C (PI-PLC) Enzyme from Streptomyces antibioticus

Released:
DOI: 10.2210/pdb3h4w/pdb
PDB entry 3h4w coloured by chain, front view.
PDB entry 3h4w coloured by chain, side view.
PDB entry 3h4w coloured by chain, top view.
Source organism: Streptomyces antibioticus
Entry authors: Jackson MR, Selby TL

Function and Biology Details

Reaction catalysed: 
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H(2)O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109787 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphatidylinositol-specific phospholipase C1 Chain: A
Molecule details ›
Chain: A
Length: 339 amino acids
Theoretical weight: 36.87 KDa
Source organism: Streptomyces antibioticus
Expression system: Escherichia coli
UniProt:
  • Canonical: B3A043 (Residues: 27-344; Coverage: 100%)
Gene name: plc1
Sequence domains: Phosphoinositide phospholipase C, Ca2+-dependent
Structure domains: Phosphatidylinositol (PI) phosphodiesterase

Ligands and Environments

4 bound ligands:
Ligand CL 1 x CL
Ligand EOH 8 x EOH
Ligand ACE 2 x ACE
Ligand GOL 3 x GOL
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21212
Unit cell:
a: 52.377Å b: 155.172Å c: 41.409Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.184 0.2
Expression system: Escherichia coli

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