› 3h5i

X-ray diffraction
1.9Å resolution

Crystal structure of the N-terminal domain of a response regulator/sensory box/GGDEF 3-domain protein from Carboxydothermus hydrogenoformans

Released:
DOI: 10.2210/pdb3h5i/pdb
PDB entry 3h5i coloured by chain, front view.
PDB entry 3h5i coloured by chain, side view.
PDB entry 3h5i coloured by chain, top view.
Source organism: Carboxydothermus hydrogenoformans Z-2901
Entry authors: Bonanno JB, Gilmore M, Bain KT, Iizuka M, Romero R, Wasserman S, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174505 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Stage 0 sporulation protein A homolog Chain: A
Molecule details ›
Chain: A
Length: 140 amino acids
Theoretical weight: 15.52 KDa
Source organism: Carboxydothermus hydrogenoformans Z-2901
Expression system: Escherichia coli
UniProt:
  • Canonical: Q3ADQ4 (Residues: 2-130; Coverage: 19%)
Gene name: CHY_0880
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand NA 1 x NA
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: R32
Unit cell:
a: 86.668Å b: 86.668Å c: 131.156Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.213 0.212 0.238
Expression system: Escherichia coli

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