› 3h5n

X-ray diffraction
1.9Å resolution

Crystal structure of E. coli MccB + ATP

Released:
DOI: 10.2210/pdb3h5n/pdb
PDB entry 3h5n coloured by chain, front view.
PDB entry 3h5n coloured by chain, side view.
PDB entry 3h5n coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
How the MccB bacterial ancestor of ubiquitin E1 initiates biosynthesis of the microcin C7 antibiotic.
Regni CA, Roush RF, Miller DJ, Nourse A, Walsh CT, Schulman BA
EMBO J 28 1953-64 (2009)
PMID: 19494832

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175247 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
THIF-type NAD/FAD binding fold domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 353 amino acids
Theoretical weight: 39.41 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q47506 (Residues: 1-350; Coverage: 100%)
Gene name: mccB
Sequence domains: ThiF family
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ZN 4 x ZN
Ligand MG 4 x MG
Ligand ATP 4 x ATP
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 55.847Å b: 138.239Å c: 80.224Å
α: 90° β: 92.62° γ: 90°
R-values:
R R work R free
0.207 0.204 0.256
Expression system: Escherichia coli

Quick links

Citations

10 review citations

YcaO-Dependent Posttranslational Amide Activation: Biosynthesis, Structure, and Function.
Burkhart et al. (2017)
9 more

3 mentions without citation

The cyanobactin heterocyclase enzyme: a processive adenylase that operates with a defined order of reaction.
Koehnke et al. (2013)
2 more