› 3h6j

X-ray diffraction
1.6Å resolution

Crystal structure of a putative neuraminidase from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb3h6j/pdb
PDB entry 3h6j coloured by chain, front view.
PDB entry 3h6j coloured by chain, side view.
PDB entry 3h6j coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Crystal structures of respiratory pathogen neuraminidases.
Hsiao YS, Parker D, Ratner AJ, Prince A, Tong L
Biochem Biophys Res Commun 380 467-71 (2009)
PMID: 19284989

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192217 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sialidase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 438 amino acids
Theoretical weight: 47.16 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9L6G4 (Residues: 1-438; Coverage: 100%)
Gene name: DT376_07370
Sequence domains: BNR repeat-like domain
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P213
Unit cell:
a: 126.34Å b: 126.34Å c: 126.34Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.172 0.191
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Influenza neuraminidase: a druggable target for natural products.
Grienke et al. (2012)
4 more