› 3h6z

X-ray diffraction
2.8Å resolution

Crystal Structure of the Four MBT Repeats of Drosophila melanogaster Sfmbt in Complex with Peptide RHR (me)K VLR

Released:
DOI: 10.2210/pdb3h6z/pdb
PDB entry 3h6z coloured by chain, front view.
PDB entry 3h6z coloured by chain, side view.
PDB entry 3h6z coloured by chain, top view.
Source organisms:
Primary publication:
Molecular recognition of histone lysine methylation by the Polycomb group repressor dSfmbt.
Grimm C, Matos R, Ly-Hartig N, Steuerwald U, Lindner D, Rybin V, Müller J, Müller CW
EMBO J 28 1965-77 (2009)
PMID: 19494831

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163607 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Polycomb protein Sfmbt Chains: A, B
Molecule details ›
Chains: A, B
Length: 447 amino acids
Theoretical weight: 50.37 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9VK33 (Residues: 535-977; Coverage: 36%)
Gene names: CG16975, Sfmbt
Sequence domains: mbt repeat
Structure domains: SH3 type barrels.
'HR(MLZ)VLR Chains: L, M
Molecule details ›
Chains: L, M
Length: 6 amino acids
Theoretical weight: 825 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, FRU
Carbohydrate polymer
No bound ligands
1 modified residue:
Ligand MLZ 2 x MLZ

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Unit cell:
a: 70.8Å b: 97.04Å c: 214.12Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.221 0.254
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

10 review citations

Polycomb group proteins: multi-faceted regulators of somatic stem cells and cancer.
Sauvageau et al. (2010)
9 more

4 mentions without citation

Are we there yet? Initial targeting of the Male-Specific Lethal and Polycomb group chromatin complexes in Drosophila.
McElroy et al. (2014)
3 more