› 3h75

X-ray diffraction
1.6Å resolution

Crystal Structure of a Periplasmic Sugar-binding protein from the Pseudomonas fluorescens

Released:
DOI: 10.2210/pdb3h75/pdb
PDB entry 3h75 coloured by chain, front view.
PDB entry 3h75 coloured by chain, side view.
PDB entry 3h75 coloured by chain, top view.
Source organism: Pseudomonas protegens Pf-5
Entry authors: Kumaran D, Mahmood A, Burley SK, Swaminathan S, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175611 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Periplasmic binding protein domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 350 amino acids
Theoretical weight: 39.64 KDa
Source organism: Pseudomonas protegens Pf-5
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4KAG7 (Residues: 23-361; Coverage: 100%)
Gene name: PFL_3664
Sequence domains: Periplasmic binding protein domain
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 7 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21212
Unit cell:
a: 65.526Å b: 81.275Å c: 63.082Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.208 0.23
Expression system: Escherichia coli

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