› 3h7o

X-ray diffraction
1.85Å resolution

Crystal structure of scabies mite inactivated protease paralogue S-I1 (SMIPP-S-I1)

Released:
DOI: 10.2210/pdb3h7o/pdb
PDB entry 3h7o coloured by chain, front view.
PDB entry 3h7o coloured by chain, side view.
PDB entry 3h7o coloured by chain, top view.
Source organism: Sarcoptes scabiei
Primary publication:
Structural mechanisms of inactivation in scabies mite serine protease paralogues.
Fischer K, Langendorf CG, Irving JA, Reynolds S, Willis C, Beckham S, Law RH, Yang S, Bashtannyk-Puhalovich TA, McGowan S, Whisstock JC, Pike RN, Kemp DJ, Buckle AM
J Mol Biol 390 635-45 (2009)
PMID: 19427318

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180468 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidase S1 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 228 amino acids
Theoretical weight: 25.26 KDa
Source organism: Sarcoptes scabiei
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q6VPT6 (Residues: 29-256; Coverage: 99%)
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

2 bound ligands:
Ligand SO4 4 x SO4
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 47.415Å b: 80.578Å c: 114.693Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.186 0.22
Expression system: Komagataella pastoris

Quick links

Citations

5 review citations

A review of Sarcoptes scabiei: past, present and future.
Arlian et al. (2017)
4 more

1 mention without citation

Structural comparison strengthens the higher-order classification of proteases related to chymotrypsin.
Mönttinen et al. (2019)