› 3h84

X-ray diffraction
2.3Å resolution

Crystal structure of GET3

Released:
DOI: 10.2210/pdb3h84/pdb
PDB entry 3h84 coloured by chain, front view.
PDB entry 3h84 coloured by chain, side view.
PDB entry 3h84 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The crystal structures of yeast Get3 suggest a mechanism for tail-anchored protein membrane insertion.
Hu J, Li J, Qian X, Denic V, Sha B
PLoS One 4 e8061 (2009)
PMID: 19956640

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-171306 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATPase GET3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 354 amino acids
Theoretical weight: 39.39 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12154 (Residues: 1-354; Coverage: 100%)
Gene names: ARR4, D2371, GET3, YDL100C
Sequence domains: Anion-transporting ATPase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

4 bound ligands:
Ligand ZN 1 x ZN
Ligand MG 2 x MG
Ligand NA 3 x NA
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21212
Unit cell:
a: 219.4Å b: 113.743Å c: 48.37Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.222 0.247
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Tail-anchored membrane protein insertion into the endoplasmic reticulum.
Hegde et al. (2011)
11 more

14 mentions without citation

The complex process of GETting tail-anchored membrane proteins to the ER.
Chartron et al. (2012)
13 more