3h8d

X-ray diffraction
2.2Å resolution

Crystal structure of Myosin VI in complex with Dab2 peptide

Released:
Source organisms:
Primary publication:
Myosin VI undergoes cargo-mediated dimerization.
Cell 138 537-48 (2009)
PMID: 19665975

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-131584 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Unconventional myosin-VI Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 141 amino acids
Theoretical weight: 16.41 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q64331 (Residues: 1134-1262; Coverage: 10%)
Gene names: Myo6, Sv
Sequence domains: Myosin VI cargo binding domain
Disabled homolog 2 Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 48 amino acids
Theoretical weight: 4.99 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O88797 (Residues: 675-713; Coverage: 5%)
Gene names: Dab2, Doc2

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P21
Unit cell:
a: 69.177Å b: 69.952Å c: 78.537Å
α: 90° β: 98.02° γ: 90°
R-values:
R R work R free
0.192 0.189 0.251
Expression system: Escherichia coli BL21(DE3)