› 3h8s

X-ray diffraction
2.51Å resolution

Structure of D19N T4 RNase H in the presence of divalent magnesium

Released:
DOI: 10.2210/pdb3h8s/pdb
PDB entry 3h8s coloured by chain, front view.
PDB entry 3h8s coloured by chain, side view.
PDB entry 3h8s coloured by chain, top view.
Source organism: Escherichia virus T4
Entry authors: Tomanicek SJ, Mueser TC

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146542 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease H Chain: A
Molecule details ›
Chain: A
Length: 305 amino acids
Theoretical weight: 35.61 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli
UniProt:
  • Canonical: P13319 (Residues: 1-305; Coverage: 100%)
Gene names: 33.2, das, rnh
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P212121
Unit cell:
a: 53.312Å b: 75.298Å c: 88.09Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.234
Expression system: Escherichia coli

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