› 3h94

X-ray diffraction
3.84Å resolution

Crystal structure of the membrane fusion protein CusB from Escherichia coli

Released:
DOI: 10.2210/pdb3h94/pdb
PDB entry 3h94 coloured by chain, front view.
PDB entry 3h94 coloured by chain, side view.
PDB entry 3h94 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Crystal structure of the membrane fusion protein CusB from Escherichia coli.
Su CC, Yang F, Long F, Reyon D, Routh MD, Kuo DW, Mokhtari AK, Van Ornam JD, Rabe KL, Hoy JA, Lee YJ, Rajashankar KR, Yu EW
J Mol Biol 393 342-55 (2009)
PMID: 19695261

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160138 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cation efflux system protein CusB Chains: A, B
Molecule details ›
Chains: A, B
Length: 407 amino acids
Theoretical weight: 44.35 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P77239 (Residues: 1-407; Coverage: 100%)
Gene names: JW0563, b0574, cusB, ylcD
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand AG 2 x AG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: I222
Unit cell:
a: 85.005Å b: 114.418Å c: 259.08Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.28 0.28 0.3
Expression system: Escherichia coli

Quick links

Citations

19 review citations

Bacterial multidrug efflux transporters.
Delmar et al. (2014)
18 more

8 mentions without citation

The Cus efflux system removes toxic ions via a methionine shuttle.
Su et al. (2011)
7 more