› 3h9w

X-ray diffraction
1.9Å resolution

Crystal Structure of the N-terminal domain of Diguanylate cyclase with PAS/PAC sensor (Maqu_2914) from Marinobacter aquaeolei, Northeast Structural Genomics Consortium Target MqR66C

Released:
DOI: 10.2210/pdb3h9w/pdb
PDB entry 3h9w coloured by chain, front view.
PDB entry 3h9w coloured by chain, side view.
PDB entry 3h9w coloured by chain, top view.
Source organism: Marinobacter nauticus VT8
Entry authors: Seetharaman J, Su M, Wang H, Foote EL, Mao L, Nair R, Rost B, Acton TB, Xiao R, Everett JK, Montelione GT, Tong L, Hunt JF, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106722 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
diguanylate cyclase Chain: A
Molecule details ›
Chain: A
Length: 115 amino acids
Theoretical weight: 13.81 KDa
Source organism: Marinobacter nauticus VT8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A1U4R9 (Residues: 22-127; Coverage: 34%)
Gene name: Maqu_2914
Sequence domains: PAS fold
Structure domains: PAS domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P6522
Unit cell:
a: 55.531Å b: 55.531Å c: 136.238Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.196 0.221
Expression system: Escherichia coli BL21(DE3)

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