› 3han

X-ray diffraction
2.75Å resolution

Crystal structure of bacteriorhodopsin mutant V49A crystallized from bicelles

Released:
DOI: 10.2210/pdb3han/pdb
PDB entry 3han coloured by chain, front view.
PDB entry 3han coloured by chain, side view.
PDB entry 3han coloured by chain, top view.
Source organism: Halobacterium salinarum
Primary publication:
Similar energetic contributions of packing in the core of membrane and water-soluble proteins.
Joh NH, Oberai A, Yang D, Whitelegge JP, Bowie JU
J Am Chem Soc 131 10846-7 (2009)
PMID: 19603754

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136411 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriorhodopsin Chain: A
Molecule details ›
Chain: A
Length: 249 amino acids
Theoretical weight: 26.9 KDa
Source organism: Halobacterium salinarum
Expression system: Halobacterium salinarum
UniProt:
  • Canonical: P02945 (Residues: 14-262; Coverage: 95%)
Gene names: VNG_1467G, bop
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

1 bound ligand:
Ligand RET 1 x RET
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2221
Unit cell:
a: 44.948Å b: 102.878Å c: 128.422Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.222 0.282
Expression system: Halobacterium salinarum

Quick links

Citations

12 review citations

Lessons from the lysozyme of phage T4.
Baase et al. (2010)
11 more

1 mention without citation

A chemical interpretation of protein electron density maps in the worldwide protein data bank.
Yao et al. (2020)