› 3hb9

X-ray diffraction
2.9Å resolution

Crystal Structure of S. aureus Pyruvate Carboxylase A610T Mutant

Released:
DOI: 10.2210/pdb3hb9/pdb
PDB entry 3hb9 coloured by chain, front view.
PDB entry 3hb9 coloured by chain, side view.
PDB entry 3hb9 coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus Mu50
Primary publication:
A symmetrical tetramer for S. aureus pyruvate carboxylase in complex with coenzyme A.
Yu LP, Xiang S, Lasso G, Gil D, Valle M, Tong L
Structure 17 823-32 (2009)
PMID: 19523900

Function and Biology Details

Reaction catalysed: 
ATP + pyruvate + HCO(3)(-) = ADP + phosphate + oxaloacetate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101946 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:

Ligands and Environments


Cofactor: Ligand BTI 4 x BTI
2 bound ligands:
Ligand ADP 2 x ADP
Ligand MN 4 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21
Unit cell:
a: 96.566Å b: 256.763Å c: 126.485Å
α: 90° β: 109.65° γ: 90°
R-values:
R R work R free
0.222 0.22 0.266
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Structure and function of biotin-dependent carboxylases.
Tong L. (2013)
6 more