› 3hba

X-ray diffraction
2Å resolution

Crystal structure of a putative phosphosugar isomerase (sden_2705) from shewanella denitrificans os217 at 2.00 A resolution

Released:
DOI: 10.2210/pdb3hba/pdb
PDB entry 3hba coloured by chain, front view.
PDB entry 3hba coloured by chain, side view.
PDB entry 3hba coloured by chain, top view.
Source organism: Shewanella denitrificans OS217
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171482 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SIS domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 334 amino acids
Theoretical weight: 35.72 KDa
Source organism: Shewanella denitrificans OS217
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12KP2 (Residues: 1-333; Coverage: 100%)
Gene name: Sden_2705
Sequence domains: SIS domain
Structure domains: Glucose-6-phosphate isomerase like protein; domain 1

Ligands and Environments

2 bound ligands:
Ligand CIT 2 x CIT
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 74.579Å b: 89.445Å c: 109.497Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.165 0.203
Expression system: Escherichia coli

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