› 3hbc

X-ray diffraction
2.27Å resolution

Crystal Structure of Choloylglycine Hydrolase from Bacteroides thetaiotaomicron VPI

Released:
DOI: 10.2210/pdb3hbc/pdb
PDB entry 3hbc coloured by chain, front view.
PDB entry 3hbc coloured by chain, side view.
PDB entry 3hbc coloured by chain, top view.
Source organism: Bacteroides thetaiotaomicron
Entry authors: Kim Y, Bigelow L, Buck K, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183707 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Choloylglycine hydrolase/NAAA C-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 320 amino acids
Theoretical weight: 36.38 KDa
Source organism: Bacteroides thetaiotaomicron
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8A600 (Residues: 25-342; Coverage: 97%)
Gene name: BT_2086
Sequence domains: Linear amide C-N hydrolases, choloylglycine hydrolase family
Structure domains: Penicillin V Acylase; Chain A

Ligands and Environments

2 bound ligands:
Ligand GOL 4 x GOL
Ligand EDO 4 x EDO
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: F222
Unit cell:
a: 74.281Å b: 135.208Å c: 167.589Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.191 0.232
Expression system: Escherichia coli BL21

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