› 3hbg

X-ray diffraction
1.9Å resolution

Structure of recombinant Chicken Liver Sulfite Oxidase mutant C185S

Released:
DOI: 10.2210/pdb3hbg/pdb
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PDB entry 3hbg coloured by chain, front view.
PDB entry 3hbg coloured by chain, side view.
PDB entry 3hbg coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
The structures of the C185S and C185A mutants of sulfite oxidase reveal rearrangement of the active site.
Qiu JA, Wilson HL, Pushie MJ, Kisker C, George GN, Rajagopalan KV
Biochemistry 49 3989-4000 (2010)
PMID: 20356030

Function and Biology Details

Reaction catalysed: 
Sulfite + O(2) + H(2)O = sulfate + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139791 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sulfite oxidase Chain: A
Molecule details ›
Chain: A
Length: 466 amino acids
Theoretical weight: 50.99 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P07850 (Residues: 1-459; Coverage: 100%)
Gene name: SUOX
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand MTE 1 x MTE
1 bound ligand:
Ligand MOM 1 x MOM
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 22-BM
Spacegroup: C2221
Unit cell:
a: 86.468Å b: 131.517Å c: 98.884Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.176 0.196
Expression system: Escherichia coli

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Citations

2 review citations

The mononuclear molybdenum enzymes.
Hille et al. (2014)
1 more

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