› 3hch

X-ray diffraction
2.1Å resolution

Structure of the C-terminal domain (MsrB) of Neisseria meningitidis PilB (complex with substrate)

Released:
DOI: 10.2210/pdb3hch/pdb
PDB entry 3hch coloured by chain, front view.
PDB entry 3hch coloured by chain, side view.
PDB entry 3hch coloured by chain, top view.
Source organism: Neisseria meningitidis serogroup A
Primary publication:
Methionine sulfoxide reductase B displays a high level of flexibility.
Ranaivoson FM, Neiers F, Kauffmann B, Boschi-Muller S, Branlant G, Favier F
J Mol Biol 394 83-93 (2009)
PMID: 19733575

Function and Biology Details

Reactions catalysed: 
L-methionine + thioredoxin disulfide + H(2)O = L-methionine (S)-S-oxide + thioredoxin
Peptide-L-methionine + thioredoxin disulfide + H(2)O = peptide-L-methionine (R)-S-oxide + thioredoxin
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191668 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptide methionine sulfoxide reductase MsrA/MsrB Chains: A, B
Molecule details ›
Chains: A, B
Length: 146 amino acids
Theoretical weight: 16.37 KDa
Source organism: Neisseria meningitidis serogroup A
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9JWM8 (Residues: 377-522; Coverage: 28%)
Gene names: NMA0290, msrAB, pilB
Sequence domains: SelR domain
Structure domains: Peptide methionine sulfoxide reductase.

Ligands and Environments

4 bound ligands:
Ligand RSM 3 x RSM
Ligand CIT 1 x CIT
Ligand P6G 5 x P6G
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P4332
Unit cell:
a: 154.879Å b: 154.879Å c: 154.879Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.196 0.226
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Oxidative stress, protein damage and repair in bacteria.
Ezraty et al. (2017)
6 more

3 mentions without citation

Molecular Mechanisms of the Methionine Sulfoxide Reductase System from Neisseria meningitidis.
Boschi-Muller S. (2018)
2 more