› 3hcs

X-ray diffraction
2.2Å resolution

Crystal structure of the N-terminal domain of TRAF6

Released:
DOI: 10.2210/pdb3hcs/pdb
PDB entry 3hcs coloured by chain, front view.
PDB entry 3hcs coloured by chain, side view.
PDB entry 3hcs coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
E2 interaction and dimerization in the crystal structure of TRAF6.
Yin Q, Lin SC, Lamothe B, Lu M, Lo YC, Hura G, Zheng L, Rich RL, Campos AD, Myszka DG, Lenardo MJ, Darnay BG, Wu H
Nat Struct Mol Biol 16 658-66 (2009)
PMID: 19465916

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195247 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
TNF receptor-associated factor 6 Chains: A, B
Molecule details ›
Chains: A, B
Length: 170 amino acids
Theoretical weight: 19.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y4K3 (Residues: 50-211; Coverage: 31%)
Gene names: RNF85, TRAF6
Sequence domains:
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)

Ligands and Environments

1 bound ligand:
Ligand ZN 10 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2
Unit cell:
a: 123.663Å b: 80.757Å c: 50.723Å
α: 90° β: 91.36° γ: 90°
R-values:
R R work R free
0.229 0.229 0.271
Expression system: Escherichia coli

Quick links

Citations

57 review citations

Building ubiquitin chains: E2 enzymes at work.
Ye et al. (2009)
56 more

23 mentions without citation

Structural studies of NF-κB signaling.
Zheng et al. (2011)
22 more