› 3hcy

X-ray diffraction
2.8Å resolution

The crystal structure of the domain of putative two-component sensor histidine kinase protein from Sinorhizobium meliloti 1021

Released:
DOI: 10.2210/pdb3hcy/pdb
PDB entry 3hcy coloured by chain, front view.
PDB entry 3hcy coloured by chain, side view.
PDB entry 3hcy coloured by chain, top view.
Source organism: Sinorhizobium meliloti 1021
Entry authors: Zhang R, Xu X, Cui H, Savchenko A, Edwards A, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187935 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Blue-light-activated histidine kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 151 amino acids
Theoretical weight: 16.91 KDa
Source organism: Sinorhizobium meliloti 1021
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q92UP1 (Residues: 292-439; Coverage: 16%)
Gene names: SM_b20933, exsG
Sequence domains: GAF domain
Structure domains: Beta-Lactamase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P6122
Unit cell:
a: 73.346Å b: 73.346Å c: 257.258Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 0.207 0.26
Expression system: Escherichia coli BL21

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