› 3hd0

X-ray diffraction
2.7Å resolution

Crystal structure of Tm1865, an Endonuclease V from Thermotoga Maritima

Released:
DOI: 10.2210/pdb3hd0/pdb
PDB entry 3hd0 coloured by chain, front view.
PDB entry 3hd0 coloured by chain, side view.
PDB entry 3hd0 coloured by chain, top view.
Source organism: Thermotoga maritima
Entry authors: Utepbergenov D, Cooper DR, Derewenda U, Derewenda ZS, Integrated Center for Structure and Function Innovation (ISFI)

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage at apurinic or apyrimidinic sites to products with a 5'-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194896 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endonuclease V Chains: A, B, D
Molecule details ›
Chains: A, B, D
Length: 237 amino acids
Theoretical weight: 27.33 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9X2H9 (Residues: 1-222; Coverage: 99%)
Gene names: TM_1865, nfi
Sequence domains: Endonuclease V
Structure domains: archaeoglobus fulgidus dsm 4304 superfamily

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 15 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 69.571Å b: 71.966Å c: 120.266Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.163 0.255
Expression system: Escherichia coli BL21

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