› 3he0

X-ray diffraction
2.2Å resolution

The Structure of a Putative Transcriptional Regulator TetR Family Protein from Vibrio parahaemolyticus.

Released:
DOI: 10.2210/pdb3he0/pdb
PDB entry 3he0 coloured by chain, front view.
PDB entry 3he0 coloured by chain, side view.
PDB entry 3he0 coloured by chain, top view.
Source organism: Vibrio parahaemolyticus
Entry authors: Cuff ME, Hendricks R, Moy S, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183380 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH tetR-type domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 196 amino acids
Theoretical weight: 22.18 KDa
Source organism: Vibrio parahaemolyticus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q87TM9 (Residues: 1-193; Coverage: 100%)
Gene name: VP0040
Sequence domains:
Structure domains: Tetracycline Repressor, domain 2

Ligands and Environments

3 bound ligands:
Ligand SO4 7 x SO4
Ligand GOL 6 x GOL
Ligand CL 2 x CL
1 modified residue:
Ligand MSE 15 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2221
Unit cell:
a: 70.272Å b: 95.548Å c: 190.541Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.187 0.227
Expression system: Escherichia coli BL21

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