3hf3

X-ray diffraction
2.2Å resolution

Old Yellow Enzyme from Thermus scotoductus SA-01

Released:

Function and Biology Details

Reaction catalysed:
NADPH + acceptor = NADP(+) + reduced acceptor
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-109157 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 349 amino acids
Theoretical weight: 38.03 KDa
Source organism: Thermus scotoductus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B0JDW3 (Residues: 1-349; Coverage: 100%)
Gene name: chrR
Sequence domains: NADH:flavin oxidoreductase / NADH oxidase family
Structure domains: Aldolase class I

Ligands and Environments


Cofactor: Ligand FMN 4 x FMN
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P2221
Unit cell:
a: 101.78Å b: 101.88Å c: 180.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.174 0.214
Expression system: Escherichia coli BL21(DE3)