› 3hfv

X-ray diffraction
2.6Å resolution

Crystal structure of human mitochondrial phenylalanyl-tRNA synthetase complexed with m-tyrosine

Released:
DOI: 10.2210/pdb3hfv/pdb
PDB entry 3hfv coloured by chain, front view.
PDB entry 3hfv coloured by chain, side view.
PDB entry 3hfv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Eukaryotic cytosolic and mitochondrial phenylalanyl-tRNA synthetases catalyze the charging of tRNA with the meta-tyrosine.
Klipcan L, Moor N, Kessler N, Safro MG
Proc Natl Acad Sci U S A 106 11045-8 (2009)
PMID: 19549855

Function and Biology Details

Reaction catalysed: 
ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131898 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phenylalanine--tRNA ligase, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 415 amino acids
Theoretical weight: 48.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O95363 (Residues: 38-451; Coverage: 92%)
Gene names: FARS1, FARS2, HSPC320
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MTY 1 x MTY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 54.64Å b: 90.143Å c: 97.133Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.192 0.239
Expression system: Escherichia coli

Quick links

Citations

12 review citations

New insights into the shikimate and aromatic amino acids biosynthesis pathways in plants.
Tzin et al. (2010)
11 more

2 mentions without citation

Eukaryotic cytosolic and mitochondrial phenylalanyl-tRNA synthetases catalyze the charging of tRNA with the meta-tyrosine.
Klipcan et al. (2009)
1 more