› 3hfx

X-ray diffraction
3.15Å resolution

Crystal structure of carnitine transporter

Released:
DOI: 10.2210/pdb3hfx/pdb
PDB entry 3hfx coloured by chain, front view.
PDB entry 3hfx coloured by chain, side view.
PDB entry 3hfx coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Crystal structure of the carnitine transporter and insights into the antiport mechanism.
Tang L, Bai L, Wang WH, Jiang T
Nat Struct Mol Biol 17 492-6 (2010)
PMID: 20357772

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152034 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-carnitine/gamma-butyrobetaine antiporter Chain: A
Molecule details ›
Chain: A
Length: 504 amino acids
Theoretical weight: 56.62 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P31553 (Residues: 1-504; Coverage: 100%)
Gene names: JW0039, b0040, caiT, yaaP
Sequence domains: BCCT, betaine/carnitine/choline family transporter

Ligands and Environments

2 bound ligands:
Ligand HG 9 x HG
Ligand 152 4 x 152
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P63
Unit cell:
a: 134.21Å b: 134.21Å c: 85Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.262 0.262 0.281
Expression system: Escherichia coli

Quick links

Citations

20 review citations

Shared Molecular Mechanisms of Membrane Transporters.
Drew et al. (2016)
19 more

8 mentions without citation

Investigation of the sodium-binding sites in the sodium-coupled betaine transporter BetP.
Khafizov et al. (2012)
7 more