› 3hg0

X-ray diffraction
2.1Å resolution

Crystal structure of a DARPin in complex with ORF49 from Lactococcal phage TP901-1

Released:
DOI: 10.2210/pdb3hg0/pdb
PDB entry 3hg0 coloured by chain, front view.
PDB entry 3hg0 coloured by chain, side view.
PDB entry 3hg0 coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure and function of a DARPin neutralizing inhibitor of lactococcal phage TP901-1: comparison of DARPin and camelid VHH binding mode.
Veesler D, Dreier B, Blangy S, Lichière J, Tremblay D, Moineau S, Spinelli S, Tegoni M, Plückthun A, Campanacci V, Cambillau C
J Biol Chem 284 30718-26 (2009)
PMID: 19740746

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163634 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
BPP Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 169 amino acids
Theoretical weight: 17.99 KDa
Source organism: Lactococcus phage TP901-1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9G096 (Residues: 1-163; Coverage: 100%)
Gene name: bpp
Sequence domains:
Structure domains: Phage tail base-plate Siphoviridae RBP, head domain
Designed Ankyrin Repeat Protein (DARPin) 20 Chain: D
Molecule details ›
Chain: D
Length: 136 amino acids
Theoretical weight: 14.74 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Ankyrin repeat-containing domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 46.65Å b: 80.44Å c: 182.87Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.208 0.243
Expression system: Escherichia coli

Quick links

Citations

16 review citations

Designed ankyrin repeat proteins (DARPins): binding proteins for research, diagnostics, and therapy.
Plückthun A. (2015)
15 more

4 mentions without citation

Structural insights for engineering binding proteins based on non-antibody scaffolds.
Gilbreth et al. (2012)
3 more