› 3hg6

X-ray diffraction
1.7Å resolution

Crystal Structure of the Recombinant Onconase from Rana pipiens

Released:
DOI: 10.2210/pdb3hg6/pdb
PDB entry 3hg6 coloured by chain, front view.
PDB entry 3hg6 coloured by chain, side view.
PDB entry 3hg6 coloured by chain, top view.
Source organism: Lithobates pipiens
Primary publication:
Three-state thermal unfolding of onconase.
Casares-Atienza S, Weininger U, Cámara-Artigas A, Balbach J, Garcia-Mira MM
Biophys Chem 159 267-74 (2011)
PMID: 21840114

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149514 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein P-30 Chain: A
Molecule details ›
Chain: A
Length: 104 amino acids
Theoretical weight: 11.85 KDa
Source organism: Lithobates pipiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P22069 (Residues: 2-104; Coverage: 99%)
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

2 bound ligands:
Ligand SO4 4 x SO4
Ligand GOL 1 x GOL
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM16
Spacegroup: P212121
Unit cell:
a: 32.516Å b: 39.799Å c: 68.536Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.185 0.237
Expression system: Escherichia coli

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Citations

1 review citation

Stability and folding of amphibian ribonuclease A superfamily members in comparison with mammalian homologues.
Arnold U. (2014)
2 other articles