› 3hh1

X-ray diffraction
1.85Å resolution

The Structure of a Tetrapyrrole methylase family protein domain from Chlorobium tepidum TLS

Released:
DOI: 10.2210/pdb3hh1/pdb
PDB entry 3hh1 coloured by chain, front view.
PDB entry 3hh1 coloured by chain, side view.
PDB entry 3hh1 coloured by chain, top view.
Source organism: Chlorobaculum tepidum
Entry authors: Cuff ME, Sather A, Clancy S, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + cytidine(1402) in 16S rRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine(1402) in 16S rRNA
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-185108 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal RNA small subunit methyltransferase I Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 117 amino acids
Theoretical weight: 12.45 KDa
Source organism: Chlorobaculum tepidum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8KBY6 (Residues: 7-120; Coverage: 47%)
Gene names: CT1646, rsmI
Sequence domains: Tetrapyrrole (Corrin/Porphyrin) Methylases
Structure domains: Cobalt-precorrin-4 Transmethylase; Domain 1

Ligands and Environments

2 bound ligands:
Ligand SO4 6 x SO4
Ligand EDO 1 x EDO
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 62.986Å b: 47.569Å c: 64.497Å
α: 90° β: 91° γ: 90°
R-values:
R R work R free
0.183 0.181 0.227
Expression system: Escherichia coli

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