› 3hhj

X-ray diffraction
2.1Å resolution

Crystal structure of mutator mutT from Bartonella henselae

Released:
DOI: 10.2210/pdb3hhj/pdb
PDB entry 3hhj coloured by chain, front view.
PDB entry 3hhj coloured by chain, side view.
PDB entry 3hhj coloured by chain, top view.
Source organism: Bartonella henselae str. Houston-1
Primary publication:
Structure of a Nudix hydrolase (MutT) in the Mg(2+)-bound state from Bartonella henselae, the bacterium responsible for cat scratch fever.
Buchko GW, Edwards TE, Abendroth J, Arakaki TL, Law L, Napuli AJ, Hewitt SN, Van Voorhis WC, Stewart LJ, Staker BL, Myler PJ
Acta Crystallogr Sect F Struct Biol Cryst Commun 67 1078-83 (2011)
PMID: 21904053

Function and Biology Details

Reaction catalysed: 
8-oxo-dGTP + H(2)O = 8-oxo-dGMP + diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102113 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
8-oxo-dGTP diphosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 158 amino acids
Theoretical weight: 17.81 KDa
Source organism: Bartonella henselae str. Houston-1
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H3LW43 (Residues: 1-137; Coverage: 100%)
Gene names: BH02020, mutT
Sequence domains: NUDIX domain
Structure domains: Nucleoside Triphosphate Pyrophosphohydrolase

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: C2
Unit cell:
a: 96.5Å b: 93.49Å c: 43.88Å
α: 90° β: 109.24° γ: 90°
R-values:
R R work R free
0.203 0.202 0.234
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Substrate ambiguity among the nudix hydrolases: biologically significant, evolutionary remnant, or both?
McLennan AG. (2013)
2 more

3 mentions without citation

The evolution of function within the Nudix homology clan.
Srouji et al. (2017)
2 more