› 3hhs

X-ray diffraction
1.97Å resolution

Crystal Structure of Manduca sexta prophenoloxidase

Released:
DOI: 10.2210/pdb3hhs/pdb
PDB entry 3hhs coloured by chain, front view.
PDB entry 3hhs coloured by chain, side view.
PDB entry 3hhs coloured by chain, top view.
Source organism: Manduca sexta
Primary publication:
Crystal structure of Manduca sexta prophenoloxidase provides insights into the mechanism of type 3 copper enzymes.
Li Y, Wang Y, Jiang H, Deng J
Proc Natl Acad Sci U S A 106 17002-6 (2009)
PMID: 19805072

Function and Biology Details

Reaction catalysed: 
(1a) L-tyrosine + 1/2 O(2) = L-dopa
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129186 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Phenoloxidase subunit 2 Chain: A
Molecule details ›
Chain: A
Length: 694 amino acids
Theoretical weight: 80.03 KDa
Source organism: Manduca sexta
UniProt:
  • Canonical: Q25519 (Residues: 2-695; Coverage: 100%)
Gene name: ppo
Sequence domains:
Structure domains:
Phenoloxidase subunit 1 Chain: B
Molecule details ›
Chain: B
Length: 684 amino acids
Theoretical weight: 78.93 KDa
Source organism: Manduca sexta
UniProt:
  • Canonical: O44249 (Residues: 2-685; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CU 4 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 148.832Å b: 153.664Å c: 75.763Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.154 0.194

Quick links

Citations

11 review citations

Proteolytic cascades and their involvement in invertebrate immunity.
Cerenius et al. (2010)
10 more

14 mentions without citation

Copper active sites in biology.
Solomon et al. (2014)
13 more