› 3hhw

X-ray diffraction
2.7Å resolution

Complex of a vesicular stomatitis virus empty capsid with the nucleocapsid-binding domain of the phosphoprotein

Released:
DOI: 10.2210/pdb3hhw/pdb
PDB entry 3hhw coloured by chain, front view.
PDB entry 3hhw coloured by chain, side view.
PDB entry 3hhw coloured by chain, top view.
Source organism: Vesicular stomatitis Indiana virus
Primary publication:
Structure of the vesicular stomatitis virus nucleocapsid in complex with the nucleocapsid-binding domain of the small polymerase cofactor, P.
Green TJ, Luo M
Proc Natl Acad Sci U S A 106 11713-8 (2009)
PMID: 19571006

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero icosamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138223 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Phosphoprotein Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 87 amino acids
Theoretical weight: 9.89 KDa
Source organism: Vesicular stomatitis Indiana virus
Expression system: Escherichia coli
UniProt:
  • Canonical: P04880 (Residues: 183-265; Coverage: 31%)
Gene name: P
Sequence domains: Vesiculovirus phosphoprotein
Structure domains: Vesicular stomatitis virus phosphoprotein C-terminal domain
Nucleoprotein Chains: K, L, M, N, O
Molecule details ›
Chains: K, L, M, N, O
Length: 421 amino acids
Theoretical weight: 47.43 KDa
Source organism: Vesicular stomatitis Indiana virus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q77E03 (Residues: 2-422; Coverage: 100%)
Gene name: N
Sequence domains: Rhabdovirus nucleocapsid protein
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand TAR 6 x TAR
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21212
Unit cell:
a: 170.602Å b: 234.52Å c: 95.05Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.264 0.263 0.296
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Understanding and altering cell tropism of vesicular stomatitis virus.
Hastie et al. (2013)
17 more

8 mentions without citation

Structure of the vesicular stomatitis virus nucleocapsid in complex with the nucleocapsid-binding domain of the small polymerase cofactor, P.
Green et al. (2009)
7 more