› 3hi0

X-ray diffraction
2.3Å resolution

Crystal structure of Putative exopolyphosphatase (17739545) from AGROBACTERIUM TUMEFACIENS str. C58 (Dupont) at 2.30 A resolution

Released:
DOI: 10.2210/pdb3hi0/pdb
PDB entry 3hi0 coloured by chain, front view.
PDB entry 3hi0 coloured by chain, side view.
PDB entry 3hi0 coloured by chain, top view.
Source organism: Agrobacterium fabrum str. C58
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
(Polyphosphate)(n) + H(2)O = (polyphosphate)(n-1) + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108926 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
exopolyphosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 508 amino acids
Theoretical weight: 56.23 KDa
Source organism: Agrobacterium fabrum str. C58
Expression system: Escherichia coli
UniProt:
  • Canonical: A9CJF9 (Residues: 1-507; Coverage: 100%)
Gene names: Atu1145, ppx
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MPD 2 x MPD
Ligand ACT 2 x ACT
Ligand CL 2 x CL
1 modified residue:
Ligand MSE 22 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P212121
Unit cell:
a: 56.327Å b: 66.302Å c: 329.654Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.233
Expression system: Escherichia coli

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